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目的观察白藜芦醇(RV)对慢性哮喘小鼠模型气道重塑的影响。方法 24只雌性BALB/c小鼠随机分成3组,即对照组、哮喘组、RV组,每组8只,3组小鼠均给予鸡卵清蛋白(OVA)致敏,对照组予以磷酸盐缓冲溶液(PBS)激发,哮喘组和RV组予以OVA激发,其中RV组于每次激发前30 min给予腹腔注射溶于二甲基亚砜(DMSO)的RV,对照组和哮喘组给予腹腔注射等量的DMSO。最后一次激发24 h后,取小鼠肺组织固定、切片行过碘酸希夫(PAS)染色、Masson染色及α-平滑肌肌动蛋白(α-SMA)免疫组织化学检测,分别检测杯状细胞增生、胶原沉积和平滑肌细胞增生肥大程度,结果用杯状细胞(PAS阳性)、胶原(Masson阳性)和平滑肌细胞(α-SMA阳性)阳染面积/支气管基底膜周长(μm2/μm)表示。结果对照组未见PAS+区域,哮喘组和RV组杯状细胞增生显著高于对照组(5.44±1.13、4.18±0.85比0.00±0.00,P均<0.01),且RV组显著低于哮喘组(P<0.05)。Masson染色提示哮喘组和RV组上皮下胶原沉积显著高于对照组(9.80±2.78、5.71±0.68比1.67±0.65,均P<0.01),且RV组显著低于哮喘组(P<0.01)。α-SMA免疫组化显示哮喘组和RV组上皮下胶原沉积显著高于对照组(10.39±1.65、7.57±1.98比2.41±1.06,均P<0.01),且RV组显著低于哮喘组(P<0.05)。结论 RV干预后,小鼠慢性哮喘模型气道重塑指标均有减轻,提示RV可能对慢性哮喘小鼠气道重塑具有抑制作用。
Objective To observe the effects of resveratrol (RV) on airway remodeling in a mouse model of chronic asthma. Methods Twenty-four female BALB / c mice were randomly divided into three groups: control group, asthma group and RV group. Eight mice in each group were sensitized with OVA and the control group received phosphate The rats in the asthma group and the RV group were challenged with OVA challenged with buffered saline (PBS). The RV group was given intraperitoneal injection of DMSO dissolved in dimethylsulfoxide (DMSO) 30 minutes prior to each challenge, and the control and asthma groups were intraperitoneally injected The same amount of DMSO. Twenty-four hours after the last challenge, the lung tissues of mice were fixed and stained with PAS staining, Masson staining and α-smooth muscle actin (α-SMA) immunohistochemistry. Goblet cells Hyperplasia, collagen deposition and hypertrophy of smooth muscle cells. The results were expressed in terms of goblet cells (PAS positive), collagen (Masson positive) and smooth muscle cells (α-SMA positive) area / bronchial basement membrane perimeter (μm2 / μm) . Results There was no PAS + region in the control group. The goblet cell proliferation in the asthma group and RV group was significantly higher than that in the control group (5.44 ± 1.13, 4.18 ± 0.85 vs 0.00 ± 0.00, P <0.01, respectively) P <0.05). Masson staining showed that the deposition of collagen in the asthma and RV groups was significantly higher than that in the control group (9.80 ± 2.78,5.71 ± 0.68 vs 1.67 ± 0.65, all P <0.01), and significantly lower in the RV group than in the asthma group (P <0.01). α-SMA immunohistochemistry showed that the deposition of collagen in the asthma and RV groups was significantly higher than that in the control group (10.39 ± 1.65,7.57 ± 1.98 vs 2.41 ± 1.06, both P <0.01), and the RV group was significantly lower than the asthma group (P <0.05). Conclusion After the intervention of RV, the indexes of airway remodeling in mice with chronic asthma were reduced, suggesting that RV may have an inhibitory effect on airway remodeling in chronic asthmatic mice.