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目的针对稻草直接还田需要,构建能够高效降解水稻秸秆同时又能抑制水稻纹枯病菌的多功能复合菌系。方法通过将具有高效降解纤维素的天然复合菌群与具有抑制水稻纹枯病菌效果的菌株组合,构建多功能复合菌系;采用失重法检测该复合菌系对水稻秸杆的腐解作用,荧光定量PCR法检测其对水稻纹枯病菌的抑制效果。结果成功地构建了一组多功能复合菌系,腐解12 d后,水稻秸秆干物质总失重率为41.4%,其中半纤维素降解率为59.5%,纤维素降解率为52.5%,木质素降解率为15.3%,腐解过程中平均CMC酶活为8.1 IU/g。该复合菌系对水稻纹枯病菌的抑制效果明显,发酵40 d后对水稻纹枯病菌的抑制率为27.1%,对照组抑制率为2.7%。结论该复合菌系能高效降解水稻秸秆,同时又能较好地抑制水稻纹枯病菌,适宜在水稻秸秆直接还田过程中使用。
OBJECTIVE To establish a multifunctional composite bacterial strain that can efficiently degrade rice straw and inhibit Rhizoctonia solani. Methods The multi-functional complex strains were constructed by combining the natural composite bacterial strains with high degrading cellulose and the strains with the effect of inhibiting Rhizoctonia solani. The decomposing effect of the compound strains on rice straw was tested by weight loss method. Fluorescence Quantitative PCR Method for Inhibition of Rhizoctonia solani. Results A series of multifunctional composite strains were successfully constructed. After 12 days of decomposition, the total weight loss of dry matter of rice straw was 41.4%, of which the degradation rate of hemicellulose was 59.5%, the degradation rate of cellulose was 52.5% The degradation rate was 15.3%, and the average CMCase activity during decomposing was 8.1 IU / g. The compound strain had a significant inhibitory effect on R. solani. After 40 days of fermentation, the inhibitory rate against R. solani was 27.1% and that of the control group was 2.7%. Conclusion The composite strain can efficiently degrade rice straw, and at the same time, can inhibit R. solanacearum and is suitable for direct application of rice straw.