论文部分内容阅读
目的 探索人巨细胞病毒 (HCMV )先天性感染对胎鼠胚胎生长及脑组织发育的影响。方法 10周龄的ICR♀♂鼠按 1∶1配对 ,实验组于交配前及妊娠 3天 (E3 )、7天(E7)、15天 (E15)腹腔内接种HCMV悬液 ,对照组接种HF细胞上清液。于E0 、E3 、E6、E9、E12 、E15、E19称取孕鼠体重。临产时剖腹取胚胎的大脑皮质 ,用常规组织切片及HE染色 ,检测组织的病理变化 ,同时取部分组织进行病毒分离。结果 各实验组 (除E15组 )孕鼠自E15后体重较对照组明显降低。组织病理学检查发现 ,各实验组ICR胎鼠大脑皮质血管扩张、充血、出血 ,脑实质有单核细胞浸润 ,部分神经细胞变性坏死或凋亡及噬神经细胞现象 ,软化灶形成 ;受染的神经细胞核内出现HCMV特征性包涵体。E0 、E3 、E7、E15实验组大脑皮质病毒分离阳性率分别为 6 0 %、6 2 %、6 7%、2 5 % ,组间差异显著 (χ2 =13 475 ,P <0 0 5 ) ,E15组低于其他3组。结论 不同孕期ICR胎鼠大脑皮质均可先天性实验感染HCMV且大脑皮质损伤明显 ,胚胎发育迟缓
Objective To investigate the effects of human cytomegalovirus (HCMV) congenital infection on fetal rat embryo growth and brain development. Methods 10-week-old ICR♀ mice were paired with 1: 1. The experimental group were intraperitoneally inoculated with HCMV suspension before mating and at 3 days of gestation (E3), 7 days (E7) and 15 days (E15) Cell supernatant. Pregnant rats were weighed at E0, E3, E6, E9, E12, E15, E19. At the time of labor, the cortex of the embryo was taken by cesarean section, and the histopathological changes were detected by routine histological sections and HE staining. At the same time, some tissues were taken for virus isolation. Results The body weight of pregnant rats in each experimental group (except E15 group) was significantly lower than that of the control group after E15. Histopathological examination revealed that cerebral blood vessels in the ICR fetuses in each experimental group were dilated, hyperemic and hemorrhaged, mononuclear cells infiltrated in the brain parenchyma, degeneration, necrosis or apoptosis of some nerve cells, and neuropharyngeal phenomena and softening lesions were formed. Infected HCMV characteristic inclusion bodies appear in the nucleus of neurons. The positive rates of cerebral cortex virus isolation in E0, E3, E7 and E15 experimental groups were 60%, 62%, 67% and 25%, respectively, with significant difference between the two groups (χ2 = 13 475, P 0 05) E15 group is lower than the other three groups. CONCLUSIONS: ICM fetuses in different cerebral cortex during pregnancy may be congenital HCMV infection and cerebral cortex injury significantly delayed embryonic development