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目的初步建立乙型肝炎病毒中国株全基因参照序列。方法测定７例慢性无症状ＨＢＶ携带者ＨＢＶ基因组全序列，结合已有的２株ＨＢＶ中国株全基因序列，经同源性比较及对齐比较，确定基因型和参照序列。结果血清型ａｄｒ４例，ａｄｗ３例；２例基因型为Ｂ型，余均为Ｃ型。各序列间Ｘ基因差异最大。该参照序列与国外参照序列仅有２２个位点的差异。结论拟定了中国ＨＢＶＣ基因型毒株全基因参照序列，建立不同地区的ＨＢＶ毒株参照序列尚待进一步收集各地区ＨＢＶ全基因序列。
Objective To establish a complete genome-wide reference sequence of hepatitis B virus Chinese strain. Methods The complete sequence of HBV genome in seven patients with chronic asymptomatic HBV carriers was determined. The complete genomes of two Chinese HBV isolates were obtained. The genotypes and reference sequences were determined by homology comparison and align comparison. Results Serum adr4 cases, adw3 cases; 2 cases of genotype B, the remaining are C-type. X gene among the sequences of the largest differences. The reference sequence and foreign reference sequence only 22 differences. Conclusion The complete genome reference sequence of Chinese HBVC genotype was developed. To establish reference sequences of HBV strains in different regions, it is necessary to further collect HBV whole genome sequences in all regions.