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目的:比较甘肃产柴胡不同炮制品中柴胡皂苷a的含量及其对小鼠的抗抑郁作用。方法:采用HPLC测定甘肃产柴胡不同炮制品中柴胡皂苷a含量,并通过自主活动、悬尾和强迫游泳实验及脑内5-HT及NE含量的测定,比较柴胡不同炮制品对行为绝望抑郁模型小鼠的影响。结果:甘肃产柴胡不同炮制品中柴胡皂苷a的含量以酒柴胡含量最高,鳖血柴胡次之。不同炮制品均能缩短两种行为绝望模型小鼠的不动时间,以鳖血柴胡及醋柴胡效果最明显;不同炮制品均可提高小鼠脑内5-HT含量,以鳖血柴胡效果最显著,具有显著性差异。结论:炮制可使甘肃产柴胡皂苷a含量发生变化,并改善小鼠的绝望行为,增加脑内5-HT含量而显示抗抑郁作用,有良好的开发前景。
Objective: To compare the content of saikosaponin a in different processed products of Bupleurum and its antidepressant effect on mice. Methods: The contents of saikosaponin a in different processed products of Bupleurum chinense were determined by HPLC. The contents of 5-HT and NE in brain were determined by autonomic activity, Destructive depression model in mice. Results: The contents of saikosaponin a produced by different processed products of Bupleurum in Gansu were the highest with the content of wine Bupleurum. Different processed products can shorten the two kinds of behavioral despair model mice immobility time to turtle blood Bupleurum and vinegar Bupleurum most obvious effect; different processed products can increase the brain 5-HT content in mice, Hu effect is the most significant, with significant differences. Conclusion: Processing can make the content of saikosaponin in Gansu change, and improve the mice desperation behavior, increase the brain content of 5-HT and show anti-depressant effect, have good prospects for development.