论文部分内容阅读
1案例资料1.1简要案情因怀疑胎儿非亲生,李某(男,37岁)要求对其妻血液和死胎组织(男,4月胎龄)进行DNA检验,鉴定与胎儿的亲缘关系。1.2检验采用Chelex-100法提取李某和其妻血液及胎儿组织DNA,采用Identifiler试剂盒(美国AB公司)、Sinofiler试剂盒(美国AB公司)和Yfiler试剂盒(美国AB公司)进行扩增,产物进行DNA分型。检测样本DNA模板扩增均在Mastercycle Gradient型PCR扩增仪(德国Eppendorf公司)上完成,实验条件和步骤
1 Case Information 1.1 Brief Case Because suspected fetal non-biological, Lee (male, 37 years old) asked his wife blood and fetal tissue (male, 4-month gestational age) for DNA testing to identify the genetic relationship with the fetus. 1.2 Test Chelex-100 extraction method Lee and his wife blood and fetal tissue DNA, using Identifiler Kit (AB USA), Sinofiler Kit (AB, USA) and Yfiler kit (AB USA) for amplification, The product is subjected to DNA typing. Detection of DNA template amplification samples were Mastercycle Gradient PCR amplification (Germany Eppendorf company) to complete the experimental conditions and steps