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采用流式细胞测量术（ＦＣＭ），对５７例人胃癌存档石蜡标本的原发灶和转移灶癌细胞的ＤＮＡ含量与倍体类型进行了检测，同时利用ＭｃＡｂ—ｐ５３对突变型ｐ５３基因产物进行了免疫组化检测。结果显示：①ＤＮＡ非整倍体与胃癌的肝脏转移密切相关（Ｐ＜０．０１），但与卵巢和淋巴结转移无明显相关；②突变型ｐ５３基因产物的表达也与胃癌肝转移密切相关（Ｐ＜０．０１）；③ＤＮＡ非整体倍体与胃癌组织中突变型ｐ５３基因产物的表达无明显相关（Ｐ＞０．０５）。上述结果提示：胃癌细胞的ＤＮＡ非整体倍体和突变型ｐ５３基因产物的表达在其血行转移过程中起重要作用，但作用机理可能有所不同。作者认为，对此两项指标的联合检测对预测胃癌的血行转移危险性并进行早期诊断将会有一定价值。
Flow cytometry (FCM) was used to detect the DNA content and ploidy type of primary and metastatic foci in 57 cases of human gastric cancer paraffin-embedded specimens. The McAb-p53 was used to detect the mutant p53 gene product. Immunohistochemical detection. The results showed that: 1 DNA aneuploidy was closely related to liver metastasis of gastric cancer (P<0.01), but was not significantly related to ovarian and lymph node metastasis; 2 the expression of mutant p53 gene product was also closely related to liver metastasis of gastric cancer (P <0.01);3There was no significant correlation between DNA non-holoploid and the expression of mutant p53 gene in gastric cancer tissues (P>0.05). The above results suggest that DNA non-holloploid and mutant p53 gene product expression in gastric cancer cells play an important role in the process of hematogenous metastasis, but the mechanism of action may be different. The authors believe that the joint detection of these two indicators will have some value in predicting the risk of hematogenous metastasis and early diagnosis of gastric cancer.