采用组织透明技术,研究了亲合性互作体系中新月弯孢菌在玉米叶片上的侵染过程。结果表明,接种2 h后分生孢子即可萌动,4、6 h的萌发率分别为80%和99%,12～24 h之间侵染丝从细胞间隙和气孔侵入表皮细胞,穿透细胞壁在细胞间迅速扩展。侵染丝穿透寄主细胞壁时菌丝明显变细。菌丝在穿过气孔时,气孔结构明显被破坏,保卫细胞被降解。接种3 d后,病菌从气孔或细胞间隙伸出,形成分生孢子梗和分生孢子,叶片表现明显症状。 Tissue-transparent technique was used to study the infection process of Curvularia lunata in maize leaves in the affinity interaction system. The results showed that the conidia germinated 2 h after inoculation, the germination rates at 4 and 6 h were 80% and 99%, respectively. Infected silk penetrated into the epidermal cells from the intercellular space and stomata within 12-24 h and penetrated the cell wall Rapid expansion between cells. Infected filaments penetrate the host cell wall mycelium significantly thinner. Mycelium through stomatal, stomatal structure was significantly damaged, guard cells were degraded. After 3 days of inoculation, the bacteria extended from the stomata or intercellular space to form conidiophores and conidia, and the leaves showed obvious symptoms.