论文部分内容阅读
建立了RAPD扩增条件快速优化程序与方法,并应用于天麻基因组DNA扩增条件的优化及多态性的测定;获得了天麻基因组DNA的RAPD扩增优化条件和DNA指纹图谱;分析了模板DNA、引物、dNTP、TaqDNA聚合酶等的浓度和退火温度对RAPD扩增的影响.结果表明:天麻基因组DNA用引物S1扩增的片段具有更明显的多态性,这种指纹图谱更适合于天麻遗传分化研究;而用引物S12扩增的DNA指纹图谱具有更大的相似性,这种指纹图谱更适合于天麻真伪鉴别.该方法使RAPD扩增条件优化过程实现了程序化和数量化,是获得RAPD优化条件的简便快速、经济实用方法.应用该方法进行RAPD扩增,可获得图谱清晰、稳定可靠的实验结果.
A rapid optimization program and method of RAPD amplification conditions were established and applied to the optimization of genomic DNA amplification conditions and the determination of polymorphism of Gastrodia elata Blume. The optimized conditions of RAPD amplification and DNA fingerprinting of genomic DNA were obtained. , Primer, dNTP, Taq DNA polymerase, and annealing temperature on the amplification of RAPD.The results showed that the fragment amplified by primer S1 had a more obvious polymorphism, which was more suitable for Gastrodia elata DNA fingerprinting with primer S12 has a greater similarity.This fingerprinting is more suitable for the authenticity identification of Gastrodia elata.This method makes program optimization and quantification of RAPD amplification conditions optimization, Is a simple and rapid, economical and practical method to obtain RAPD optimization conditions.Applying this method to RAPD amplification can obtain clear, stable and reliable experimental results.