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目的:制作和评价以小肠切除-吻合术模拟术后疲劳综合征(POFS)稳定的实验动物模型。方法:将42只大鼠随机分为对照组和70%、40%、10%小肠切除组。完全模拟临床手术全过程切除大鼠适量中段小肠,术后观察一般情况、进行改良悬尾试验、Morris水迷宫试验等行为学实验,并检测血清ALB、TF、PA、纤维连接蛋白(FN)来评价其术后营养状况,检测血清过氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,以评价其氧自由基水平。结果:70%小肠切除组大鼠一般情况下POFS表征符合程度最佳,体力、耐力和学习记忆力均下降,并出现一定程度的抑郁现象(P<0.05),血清蛋白水平明显下降(P<0.05),与疲劳相关的氧自由基也处于失衡状态(P<0.05)。结论:70%小肠切除-吻合术建立的大鼠动物模型,具有临床POFS的基本特征,制作方便,结果可靠,可作为研究POFS的实验动物模型。
OBJECTIVE: To make and evaluate experimental animal models of postoperative fatigue syndrome (POFS) stabilization with small bowel resection-anastomosis. Methods: Forty-two rats were randomly divided into control group and 70%, 40%, 10% small intestine resection group. Complete simulations of clinical surgery to remove the appropriate amount of the middle small intestine after surgery to observe the general situation, the modified tail suspension test, Morris water maze test and other behavioral tests, and detection of serum ALB, TF, PA, fibronectin (FN) The postoperative nutritional status was evaluated. The activity of serum superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were measured to evaluate the level of oxygen free radicals. Results: In 70% of the small intestine resection rats, POFS showed the best conformity, the physical, endurance and learning and memory abilities decreased, and a certain degree of depression (P <0.05) and serum protein level decreased significantly (P <0.05 ), And the fatigue-related oxygen free radicals are also in an imbalanced state (P <0.05). Conclusion: 70% of the small intestine resection-anastomosis rat model established with the basic features of clinical POFS, easy to produce, reliable results, can be used as a study animal model of POFS.