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通过根农杆菌介导法成功地将修饰的豇豆胰蛋白酶抑制剂（ＣｐＴＩ）基因导入新疆陆地棉栽培品种新陆早１号。棉花幼苗下胚轴经根农杆菌共培养、抗性愈伤组织筛选和胚性愈伤的诱导等阶段获得了胚状体；经ＥＬＩＳＡ检测选择Ｎｐｔ－Ⅱ阳性的克隆进行分化培养和植株再生；经ＥＬＩＳＡ再度检测，选择Ｎｐｔ－Ⅱ阳性的再生植株进一步培养。当生长至５—６片真叶时，将再生植株直接移栽到温室或进行嫁接。通过ＰＣＲ鉴定和ＰＣＲＳｏｕｔｈｅｒｎ杂交检测证明，修饰的ＣｐＴＩ基因已存在于再生植株的基因组内。抗虫测试结果表明，转化棉株对棉铃虫具有较强的抗性。
The modified cowpea trypsin inhibitor (CpTI) gene was successfully introduced into Xinjiang Upland cotton cultivars Xinluzao No.1 by Agrobacterium rhizogenes. Embryoid bodies of cotton seedling hypocotyls were co-cultured with Agrobacterium tumefaciens, screening of resistant callus and induction of embryogenic callus. Embryoid bodies were obtained by Npt-Ⅱ positive clones selected by ELISA. After re-testing by ELISA, Npt-Ⅱ positive regenerated plants were selected for further cultivation. When grown to 5-6 true leaves, the regenerated plants are transplanted directly to the greenhouse or grafted. The results of PCR and PCR Southern blot showed that the modified CpTI gene was already existed in the genome of the regenerated plant. Insect-resistant test results show that cotton plants have strong resistance to cotton bollworm.