本文介绍一种用免疫亲和层析法浓缩、提纯甲型肝炎病毒的方法。 IgG是从首次在粪便中找到甲型肝炎病毒后10周的一例病人恢复期血清中提取的。用溴化氰活化的琼脂糖珠4B 1.2ml与30mgIgG混合,可交联89％的免疫球蛋白。免疫吸附剂装在一根盘径为10mm的改良玻璃柱内。用含0.02％叠氮钠磷酸缓冲盐水冲洗平衡,再用经免疫电镜检查证明含有甲型肝 This article describes a method of using immunoaffinity chromatography to concentrate and purify the hepatitis A virus. IgG was extracted from one patient’s convalescent sera 10 weeks after the first Hepatitis A virus was found in the stool. Sepharose 4B activated with cyanogen bromide, 1.2 ml, was mixed with 30 mg IgG and 89% immunoglobulin was cross-linked. The immunoadsorbent was packed in a modified glass column with a 10 mm disk. Balanced with 0.02% sodium azide-containing phosphate buffered saline, and then confirmed by immunoelectron microscopy with hepatitis A