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用西葫芦作为罗汉果花叶病毒 (WMV 2 Luo)的繁殖寄主 ,通过差速离心和PEG二次沉淀法得到WMV 2 Luo的提纯液 ,用该病毒提纯液免疫日本大白兔 ,获得了WMV 2 Luo兔抗血清。经ELISA测定 ,其效价为 1/5 12 0 ,以 0 1mol/L磷酸盐缓冲液 (pH7 8,含 1 5 %NaDIECA)作为样品制备缓冲液 ,用间接ELISA法检测WMV 2 Luo的灵敏度为 1/32 0 0。用此方法检测罗汉果组培苗带毒情况和田间病株均获得成功。
WMV 2 Luo was purified by differential centrifugation and PEG secondary precipitation using zucchini as the host for the propagation of WMV 2 Luo, and the Japanese white rabbits were immunized with this virus extract to obtain WMV 2 Luo rabbits Antiserum. The titer was 1/5 12 0 by ELISA, and 0 1 mol / L phosphate buffer (pH7 8, containing 15% NaDIECA) was used as the sample preparation buffer. The sensitivity of indirect ELISA to detect WMV 2 Luo was 1/32 0 0. This method was used to detect the infection of Luohanguo tissue culture seedlings and field strains were successful.