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实验采用 NT 株刚地弓形虫,将含有该虫株包囊的鼠脑匀浆,分别用0.1～1.0KGy 钴~(60)丙线剂量照射,经腹腔接种小鼠后50d,用脑组织压印涂片检查弓形虫包囊和血清 IHA 试验检测抗体及盲传试验考查辐照效果。0.5KGy 及以下剂量对小鼠仍具感染性,感染率达75～25%,但弓形虫包囊的存活力随辐照剂量的增大而减弱。0.75KGy 及1.0KGy 剂量照射的包囊,对小鼠无感染性,同样经幼猫试验获得与上述相似的结果。进一步实验证明:丙线控制 NT 株弓形虫包囊感染性的最小有效剂量接近于0.55KGy。
Toxoplasma gondii of NT strain was used in experiment. The brain homogenates of mice that were infected with the strain were respectively irradiated with 0.1 ~ 1.0KGy Cobalt (60) C line and intraperitoneally inoculated with mice for 50 days. India smear test Toxoplasma cysts and serum IHA test antibody and blind pass test to test the effect of irradiation. The dose of 0.5KGy and below was still infective to mice, and the infection rate was 75% -25%. However, the viability of Toxoplasma gondii cyst decreased with the increase of irradiation dose. The cysts irradiated with 0.75KGy and 1.0KGy dose were non-infectious to mice, and similar results were obtained by the kitten test. Further experiments showed that the minimum effective dose of cyproterone cyst infestation of ciprofloxacin close to 0.55KGy.