蛋白酶活力测定的方法很多,最常用酪蛋白作底物,Folin试剂测定往往需较多时间,并且干扰因素较多.带指示基团的蛋白质也是常用作蛋白水解酶活力测定的一类底物,其中以重氮酪蛋白较广泛采用,但需在强碱溶液中比色测定,颜色的稳定性亦较差.我们在研究重 Protease activity determination of many ways, the most commonly used casein as a substrate, Folin reagent determination often takes more time and more interference factors.Proteins with the indicated group is also commonly used as a substrate for the determination of proteolytic enzyme activity, Among them diazo casein is more widely used, but the colorimetric determination of strong alkaline solution, the color stability is also poor.We are in the study of heavy