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目的：研究肝细胞癌患者经动脉化疗栓塞（ＴＡＣＥ）治疗后肿瘤的细胞凋亡与耐药基因表达的情况及两者的关 系。方法：经病理证实的肝细胞癌９８例，单纯手术５７例，４种介入治疗后Ⅱ期手术切除４１例，用流式细胞仪检测细胞凋 亡，用链霉亲合素－生物素酶复合物免疫组化检测各标本中Ｐｇｐ。结果：肿瘤细胞凋亡率和Ｐｇｐ阳性百分数在单纯手术组和 介入Ａ、Ｂ、Ｃ、Ｄ各组分别为１．７７％、６．１％、８．７％、１３．３％、１５．７％（经秩和检验，除Ｃ、Ｄ组间比较Ｐ＞０．０５，其余两两比较，Ｐ 值均＜０．０５＝和５２．６％、１００．０％、６２．５％、４６．７％、１８．２％（各介入组与单纯手术组相比，Ｘ２值分别为３．９６、０．０２、０．１７、４．３９，Ｐ 值分别＜０．０５、＞０．０５、＞０．０５、＜０．０５）。两指标改变相关系数 ｒ＝－０．９６５５，ｔ＝５．２４３，Ｐ＜０．０２５。结论：碘油、乙醇、明胶 海绵多材料栓塞细胞凋亡率增加，而耐药基因表达减少，两者呈负相关，多材料联合栓塞优于单材料栓塞和单纯化疗。
OBJECTIVE: To study the relationship between the apoptosis and drug resistance gene expression and the relationship between the two methods after transcatheter arterial chemoembolization (TACE) in patients with hepatocellular carcinoma. Methods: 98 cases of hepatocellular carcinoma confirmed by pathology, 57 cases of surgery alone, 41 cases of secondary excision after 4 interventional procedures, apoptosis detected by flow cytometry, and streptavidin-biotinase complex Immunohistochemical detection of Pgp in each specimen. Results: The percentage of tumor cell apoptosis and Pgp positive was 1.77%, 6.1%, 8.7%, 13.3%, and 15.7% in the surgery group and the intervention groups A, B, C, and D, respectively. 7% (by the rank sum test, except for C and D groups, P> 0.05, the rest were compared, the P values were <0.05 = and 52.6%, 100.0%, 62.5%, 46.7% and 18.2% (X2 values were 3.96, 0.02, 0.17, and 4.39 for each intervention group compared with the surgery alone group, P values were <0.05, >0, respectively). .05, >0.05,<0.05).The correlation coefficient between the two indexes changed r=-0.9655,t=5.243,P<0.025.Conclusion:Multi-material embolization with lipiodol,ethanol,gelatin sponge The rate of cell apoptosis increased, but the expression of drug resistance genes decreased. There was a negative correlation between the two. Multi-material combined embolization was superior to single-material embolization and chemotherapy alone.