论文部分内容阅读
1975年采得病死茶毛虫尸体。该病原被鉴定为茶毛虫核型多角体病毒(简称EPNPV),在细胞核内增殖,形成多角体,病毒束为短柱状,核衣壳为杆状,多粒包埋。核酸构型为单分子双链环状超螺旋DNA,分子量96～97×10~6道尔顿。病毒核酸内切酶图谱及蛋白结构多肽图谱分析都具有其特异性。用它的DNA探针与其他NPV-DNA杂交,鉴定出EPNPV与其他NPV-DNA的不同同源性程度。该毒株毒力生物测定,对三至五龄幼虫LG_(50)为1.18～1.58×10~5多角体/毫升。病毒增殖采用茶园治虫回收、迁集定园增殖、室内催孵催育然后罩控等简易方法,大量增殖病毒,试制了病毒粉剂、乳剂。1976年以来,于不同龄期、季节、气候、海拔、地区以3～6×10~7多角体/毫升在茶园进行试验与示范共1400多亩,杀虫率为71～97％,并观察到在茶园使用有其流行传播及持续作用。1985～1986年在福州、寿宁等地茶园推广4000多亩,杀虫率为82.9％以上,年亩有10～50元以上的经济效益(挽回损失)。对喂饲茶园优势种天敌瓢虫、蜘蛛试验无害。对实验动物的安全性试验表明,对人畜安全无害,有明显的社会效益。
In 1975, died of the body of tea caterpillars. The pathogen was identified as the coccidian nuclear polyhedrosis virus (referred to as EPNPV), in the nucleus of proliferation, the formation of polyhedrosis, the virus bundle for the short column, the nucleocapsid rod-shaped, multi-grain embedding. The nucleic acid is configured as a single-molecule double-stranded circular supercoiled DNA with a molecular weight of 96-97 × 10 -6 Daltons. Virus endonuclease map and protein structure of the peptide map analysis has its specificity. Using its DNA probe to hybridize with other NPV-DNAs, different degrees of homology of EPNPV to other NPV-DNAs were identified. The strain virulence bioassay, for three to five instar larvae LG_ (50) 1.18 ~ 1.58 × 10 ~ 5 polyhedron / ml. The proliferation of viruses using tea plant pest recovery, relocation park garden proliferation, indoor incubation and then hood control and other simple methods, a large number of proliferation of the virus trial of the virus powder, emulsion. Since 1976, 1,400 mu of plots have been tested and demonstrated in tea plantations of 3 ~ 6 × 10 ~ 7 polyhedron / ml at different ages, seasons, climates, elevations and altitudes, with insecticidal rates of 71-97% Its use in the tea plantation has its own spreading and continuing role. Between 1985 and 1986, more than 4,000 mu of tea plantations were popularized in Fuzhou and Shouning, with an insecticidal rate of over 82.9%. Annual economic benefits (recovery of losses) of 10-50 yuan per mu were obtained. The dominant species feeding tea plant ladybugs, spiders harmless. Safety tests on experimental animals show that it is safe and harmless to humans and animals and has obvious social benefits.