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To investigate the correlation between sensitivity to Fas ligand(FasL)and expression level of decoy receptor 3 (DcR3)on tumor cell surface,Fas/DcR3 mRNA expression was detected by RT-PCR.Anti-DcR3 mAb was used to detect expression level of DcR3 on surface of tumor cells by flow cytometry.Caspase-8,caspase-9,caspase-3,Bcl-2 expressions were analyzed by Western blot,respectively.Sensitivity to apoptosis induced by FasL was determined by Annexin V apoptosis kit.The expressions of DcR3 on the surface of tumor cells from high to low were approximately 35.3% in BGC823 cells,and 21.6% in MCF-7 cells,respectively.The apoptotic rates induced by FasL from low to high were 15.6% in BGC823 cells,and 58.2% in MCF-7 cells,respectively.There was a significant correlation between the expression levels of DcR3 with FasL-inducing apoptosis.
To investigate the correlation between sensitivity to Fas ligand (FasL) and expression level of decoy receptor 3 (DcR3) on tumor cell surface, Fas / DcR3 mRNA expression was detected by RT-PCR. Anti-DcR3 mAb was used to detect expression level of Sense to apoptosis induced by FasL was determined by Annexin V apoptosis kit. Expressions. DcR3 on surface of tumor cells by flow cytometry. Caspase-8, caspase-9, caspase-3, Bcl- of DcR3 on the surface of tumor cells from high to low were approximately 35.3% in BGC823 cells, and 21.6% in MCF-7 cells, respectively. apoptotic rates induced by FasL from low to high were 15.6% in BGC823 cells, and 58.2 % in MCF-7 cells, respectively. There was a significant correlation between the expression levels of DcR3 with FasL-inducing apoptosis.