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目的 :研究胶质瘤中细胞EGFR和磷酸化AKT(p AKT)的表达以及EGFR与AKT活性的相互关系。方法 :用免疫组化方法观察 6例正常脑组织、5 0例胶质瘤标本和 2个恶性胶质瘤体外细胞系的EGFR表达 ,并应用Westernblot分析p AKT的表达。结果 :EGFR在胶质瘤中的表达阳性率随肿瘤恶性程度增加而增加 ;其蛋白表达水平与病理分级呈正相关。Westernblot分析发现在正常脑组织中p AKT蛋白水平表达极低 ,胶质瘤中p AKT的表达水平均随肿瘤恶性程度增加而明显上升 ,且与胶质瘤病理级别正相关 ;低恶度肿瘤与高恶度肿瘤有明显差异。EGFR的阳性表达率与AKT磷酸化水平显著相关。结论 :EGFR AKT通路的活性状态在胶质瘤的恶性进展具有重要作用 ,该通路可能是胶质瘤恶性表型的责任通路。
OBJECTIVE: To study the expression of EGFR and phosphorylated AKT (p AKT) in gliomas and the relationship between EGFR and AKT activity. Methods: The EGFR expression in 6 normal brain tissues, 50 glioma specimens and 2 glioma cell lines were observed by immunohistochemistry. The expression of p AKT was analyzed by Western blot. Results: The positive expression rate of EGFR in glioma increased with the malignant degree of the tumor. The protein expression level was positively correlated with the pathological grade. Western blot analysis showed that the expression of p AKT protein in normal brain tissue was extremely low. The expression level of p AKT in glioma increased with the malignant degree of glioma and was positively correlated with the pathological grade of glioma. High-grade tumors have significant differences. The positive rate of EGFR was significantly correlated with AKT phosphorylation. CONCLUSION: The active status of EGFR AKT pathway plays an important role in the malignant progression of gliomas, which may be the responsible pathway of glioma malignant phenotype.