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目的建立硝酸-过氧化氢微波消解样品,原子荧光光谱法测定蕨菜、藠头、香椿、苦蒜、茴香中痕量硒的方法。方法用微波辅助消解蕨菜、藠头、香椿、苦蒜、茴香样品,氢化物发生-原子荧光光谱法测定硒的含量。结果在优化的操作条件下,硒在0 ng/ml~10.0 ng/ml范围内,线性回归方程I=91.6956C+0.8089,相关系数r=0.9999,检出限为0.011 ng/ml,相对标准偏差(n=5)小于1.5%,回收率为93.3%~102.0%。蕨菜、藠头、香椿、苦蒜、茴香中硒含量分别为78.4μg/g、72.8μg/g、95.1μg/g、69.7μg/g、100.3μg/g。结论该样品处理方法能有效地避免硒的挥发,检出限低、精密度高,是简便、快速、实用的测定食药两用植物中的硒的方法。
Objective To establish a nitric acid - hydrogen peroxide microwave digestion sample and determine the trace selenium in Brassica chinensis, Toona sinensis, Bitter garlic and fennel by atomic fluorescence spectrometry. Methods The content of selenium was determined by microwave-assisted digestion of bracken, stirrup, Chinese toon, bitter garlic, anise and hydride generation-atomic fluorescence spectrometry. Results Under optimal operating conditions, the linear regression equation I was 91.6956C + 0.8089 with a correlation coefficient of 0.9999 with a detection limit of 0.011 ng / ml for selenium in the range of 0 ng / ml to 10.0 ng / ml. The relative standard deviation (n = 5) was less than 1.5%, the recovery rate was 93.3% ~ 102.0%. The content of selenium in Bracken, Buntou, Chinese Toon, Bitter Garlic and Fennel were 78.4μg / g, 72.8μg / g, 95.1μg / g, 69.7μg / g and 100.3μg / g, respectively. Conclusion The sample preparation method can effectively avoid the volatilization of selenium and has the advantages of low detection limit and high precision. It is a simple, rapid and practical method for the determination of selenium in medicinal and medicinal plants.