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目的:观察飞扬肠胃炎胶囊对盆腔炎性后遗症大鼠的药效及其作用机制。方法:15%苯酚胶浆注入大鼠右侧子宫复制盆腔炎性后遗症动物模型,造模15d后,将动物随机分为假手术组,妇科千金胶囊组(1.2g/kg),飞扬肠胃炎胶囊低剂量组(0.6g/kg)、飞扬肠胃炎胶囊中剂量组(1.2g/kg)、飞扬肠胃炎胶囊高剂量组(2.4g/kg)和模型组,灌胃给药30d后,观察大鼠子宫外观形态变化,HE染色评价病理改变情况,ELISA检测血清中白介素1-β(IL-1β)、肿瘤坏死因子-α(TNF-α)含量,免疫组化检测子宫中核转录因子(NF-ΚB)、天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)的表达。结果:与假手术组相比,模型组大鼠血清中IL-1β、TNF-α显著升高,组织中NF-ΚB、Caspase-3表达升高,经飞扬肠胃炎胶囊中剂量(1.2g/kg)、飞扬肠胃炎胶囊高剂量(2.4g/kg)治疗后,血清中IL-1β、TNF-α含量显著降低,组织中NF-ΚB表达降低,Caspase-3表达升高。结论:飞扬肠胃炎胶囊中(1.2g/kg)、飞扬肠胃炎胶囊高剂量(2.4g/kg)对盆腔炎性后遗症大鼠有良好的治疗作用,其作用机制可能与其抑制NF-ΚB通路激活和上调凋亡通路上的Caspase-3蛋白表达有关。
Objective: To observe the effect of Fei Yang Chang Wei Capsule on pelvic inflammatory sequelae in rats and its mechanism. METHODS: Fifteen days after injection of phenol glue into the right uterus of rats, pelvic inflammatory sequelae animal models were established. After 15 days of modeling, the animals were randomly divided into sham-operation group, gynecological Qianjin capsule group (1.2g / kg) Low dose group (0.6g / kg), high dose group (1.2g / kg), high dose group (2.4g / kg) and high dose group of gavage gastroenteritis. After intragastric administration for 30 days, The morphological changes of the uterus were observed. HE staining was used to evaluate the pathological changes. Serum levels of interleukin 1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured by ELISA. The expressions of nuclear factor- ΚB), caspase-3 expression. Results: Compared with the sham group, the levels of IL-1β and TNF-α in the model group were significantly increased, the expression of NF-κB and Caspase-3 in the model group was increased, kg) and high-dose (2.4g / kg) FeiGanJingGuanYan capsule significantly reduced the levels of IL-1β and TNF-α in serum. The expression of NF-κB and the expression of Caspase-3 in the tissue were increased. Conclusion: The high-dose (2. 4g / kg) Fei-Wei Chang-Wei Capsule (1.2g / kg) has a good therapeutic effect on pelvic inflammatory sequelae in rats, and its mechanism may be related to its inhibition of NF-κB pathway activation And up-regulates the expression of Caspase-3 protein in the apoptotic pathway.