目的建立高效液相色谱法测定大黄中番泻苷A含量,为大黄质量标准化研究提供依据。方法采用高效液相色谱法,色谱柱为C18(200 mm×4.6 mm,5μm),乙腈∶水∶冰醋酸(60∶40∶10)为流动相,流速1.2 ml/min,检测波长340 nm。结果番泻苷A在0.5~2.5μg范围内具有良好的线性关系,r=0.999 7,回收率为96.7%。结论用高效液相色谱法测定中药大黄中番泻苷A含量快速、可靠、方便、准确。 Objective To establish a HPLC method for the determination of sennoside A in rhubarb and provide basis for the standardization of rhubarb quality. Methods HPLC was used. The chromatographic column was C18 (200 mm×4.6 mm, 5 μm), acetonitrile:water:acetic acid (60∶40:10) was used as the mobile phase, the flow rate was 1.2 ml/min, and the detection wavelength was 340 nm. Results There was a good linear relationship between sennoside A in the range of 0.5-2.5 μg, r=0.999, and the recovery rate was 96.7%. [Conclusion] The determination of sennoside A in rhubarb by HPLC is rapid, reliable, convenient and accurate.