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In this study,we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl,and the optimal RAPD reaction conditions were the optimal reaction mixture(25μL total volume)that contained 2.0μL 10×buffer,0.45 mmol·L~(-1) dNTPs, 2.0 mmol·L~(-1)Mg~(2+),2 U Taq DNA polymerase,0.30 umol·L~(-1)primer and 40 ng genomic DNA.Total 84 bands were amplified from 12 primers used,and the differential bands had 28 bands,which was 33% of total bands.In cluster group analysis,the four kinds of color-flowered were divided into two styles.One style is that the red color and red-white color were grouped together,then they grouped with purple color into one cluster,and the white color was another style.
In this study, we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl, and the optimal RAPD reaction conditions were the optimal reaction mixture (25 μL total volume) that contained 2.0 μL 10 × buffer, 0.45 mmol·L ~ (-1) dNTPs, 2.0 mmol·L -1 Mg 2+, 2 U Taq DNA polymerase, 0.30 umol·L -1 primer and 40 ng genomic DNA.Total 84 bands were amplified from 12 primers used, and the differential bands had 28 bands, which was 33% of total bands. In cluster group analysis, the four kinds of color-flowered were divided into two styles. One style is that the red color and red-white colors were grouped together then then grouped with purple color into one cluster, and the white color was another style.