论文部分内容阅读
采集了中国科学院桃源农业生态试验站红壤坡地农田、自然恢复林和茶园土壤样品,采用末端限制性酶切片段长度多态性分析(T-RFLP)技术分析土壤细菌、古菌、氨氧化细菌(AOB)和氨氧化古菌(AOA)的多样性,采用好气培养法测定不同土壤的硝化势,研究不同土地利用方式对微生物多样性和硝化势的影响。结果表明:土壤AOB和AOA多样性指数差异不显著,且在3种不同土地利用方式中呈现相同的趋势,均为农田=茶园>自然恢复林;通过RDA分析发现,不同利用方式造成土壤理化性状的改变是影响土壤AOA和AOB群落结构的主要原因;好气培养法测得不同土壤硝化势农田最高,茶园次之而自然恢复林最低;相关性分析显示,硝化势与细菌16S rRNA、AOA和AOB amoA基因多样性指数呈显著正相关,其中与AOA amoA基因关系最为密切;总体来说,红壤坡地不同利用方式改变了土壤细菌、古菌、AOA和AOB的多样性,土壤AOB和AOA积极参与了土壤的硝化过程,且AOA在氨氧化微生物群落生态功能中占有重要地位,AOA比AOB与硝化势的关系更为密切。
Soil samples collected from Chinese Academy of Sciences, Taoyuan Agro-ecological Experimental Station were collected from farmland, natural forest and tea plantation in red soil slopes. T-RFLP analysis of soil bacteria, archaea and ammonia oxidizing bacteria AOB and AOA. The aerobic culture method was used to determine the nitrification potential of different soils. The effects of different land use types on microbial diversity and nitrification potential were studied. The results showed that there was no significant difference in AOB and AOA index between soils and the same trends among the three land use types, which were farmland = tea plantation> natural recovery forest. According to RDA analysis, it was found that the soil physical and chemical properties Was the main reason that affected the soil AOA and AOB community structure. The aerobic culture method had the highest soil nitrification potential, the second highest tea plantation and the lowest natural recovery forest. Correlation analysis showed that nitrification potential was positively correlated with 16S rRNA, AOA and AOB amoA gene diversity index was significantly positively correlated with AOA amoA gene most closely; on the whole, red soil slope use different ways to change the soil bacteria, archaea, AOA and AOB diversity, soil AOB and AOA actively involved Soil nitrification process, and AOA plays an important role in the ecological function of ammonia oxidation microbial community. AOA is more closely related to AOB than nitrification potential.