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目的确定正常健康儿童不同年龄之间血清和脑脊液中神经元特异性烯醇化酶( )正常参考值,了解血清和脑脊液中 NSENSE 之间的变化规律,探讨影响实验结果的各种因素以及血清和脑脊中 NSE 在新生儿 H IE 时的变化及其规律。方法:应用酶联免疫法测定正常健康儿童和患有 H IE 的新生儿血清和脑脊液中 NSE 水平。结果: 例正常健康儿童血清及脑脊液中 NSE 的水平,在 66性别、年龄之间均无显著性差异( t=0.148、 , 均> 0.05);影响血清和脑脊液中 NSE 检测结果的因素包括标本的采集方法、保存 1.73 P及冻融、标本量及操作等; H IE 患儿血清及脑脊液中 NSE 与病情轻重密切相关,即 H IE 越重, NSE 升高越明显,轻、中、重三种类型H IE 三者分别相比,差异非常显著( t=2.95￣6.38, < 0.01);健康儿童和 H IE 患儿血清 NSE 水平的变化与脑脊液中 NSE 水平的变化 P成正相关( r=83.5￣87.3)。结论:儿童由于血清和脑脊液中 NSE 水平没有性别及年龄差异,故只需设置一个正常参考值;检测血清中NSE 的改变情况可以反映大脑神经元受损的情况;检测血清和脑脊液中 NSE 水平变化可以帮助判断 H IE 患儿病情的轻重,
Objective To determine the normal reference value of neuron-specific enolase in serum and cerebrospinal fluid (CSF) between children of different ages, to understand the regularity of NSENSE in serum and cerebrospinal fluid (CSF), and to explore various factors influencing experimental results, Changes and regularities of NSE in ridges during neonatal H IE. Methods: NSE levels in serum and cerebrospinal fluid of normal healthy children and newborns with H IE were determined by enzyme-linked immunosorbent assay. Results: There was no significant difference in the serum and cerebrospinal fluid levels of NSE among 66 healthy children and normal children (t = 0.148, all> 0.05). The factors affecting the NSE test results in serum and cerebrospinal fluid Collection method, save 1.73 P and freeze-thaw, specimen size and operation; NSE in children with H IE serum and cerebrospinal fluid is closely related to the severity of illness, that is, H IE heavier NSE increased more obvious, light, moderate and heavy (T = 2.95 ~ 6.38, <0.01). The changes of serum NSE levels in healthy children and children with H IE were positively correlated with the changes of NSE in cerebrospinal fluid (P = 87.3). CONCLUSIONS: Because children have no gender and age differences in serum and cerebrospinal fluid levels of NSE, only a normal reference value should be set. Detection of changes in serum NSE can reflect neuronal damage. Changes in serum and cerebrospinal fluid levels of NSE Can help determine the severity of HIE children,