通过NMR及粘度测定,本文研究了7个具有不同取代基的萘并噻吩系列化合物与DNA结合的方式。认定无论取代基的电性如何,此系列化合物均以嵌插络合的方式与DNA结合。通过光谱及热力学测量,比较了取代基的电性效应对本系列化合物与DNA结合强度的影响,发现Hammett取代基常数σ与小分子—DNA络合物的表观稳定常数K(LogK)值有一定的相关性,并据此初步探讨了其反应机制。 By means of NMR and viscosity measurement, we studied the way of seven naphthothiophene compounds with different substituents binding to DNA. This series of compounds is believed to bind to DNA by intercalation, regardless of the electrical nature of the substituent. The effect of the substituent on the DNA binding strength was investigated by spectroscopic and thermodynamic measurements. The Hammett substituent constant σ and the apparent stability constant K (LogK) of the small molecule-DNA complex were found to be certain The correlation between them, and based on the preliminary study of the reaction mechanism.