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目的:采用石墨烯(GS)-Nafion-纳米金(Au)增敏印刷电极(SPCEs)建立青藤碱(SN)的测定方法,探讨SN在修饰电极上的电化学行为。方法:将GS-Nafion溶液涂覆于SPCEs表面制得GS-Nafion︱SPCEs电极,然后于上述电极表面化学镀Au制得Au/GS-Nafion︱SPCEs电极,在B-R缓冲液中,采用循环伏安(CV)和示差脉冲伏安(DPV)法研究SN的电化学性质。结果:在优化的实验条件下,DPV氧化峰电流与SN浓度在5.0×10-7～1.0×10-4mol.L-1范围内呈良好的线性关系,检出限为2.2×10-7mol.L-1。结论:该修饰电极价格低廉,制备容易,样品用量少,抗干扰性强,可用于正清风痛宁注射液中SN含量测定。
OBJECTIVE: To establish a method for the determination of sinomenine (SN) by using graphene (GS) -Nafion-gold nanoparticle (Au) sensitized printed electrodes (SPCEs) and investigate the electrochemical behavior of SN on the modified electrode. Methods: The GS-Nafion (superscript PROCEP) / SPCEs electrode was coated with GS-Nafion solution on the surface of SPCEs. Au / GS-Nafion / SPCEs electrode was electrolessly plated on the surface of the electrode. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) method to study the electrochemical properties of SN. Results: Under the optimal experimental conditions, the oxidation peak current of DPV showed a good linear relationship with the concentration of SN in the range of 5.0 × 10-7 ~ 1.0 × 10-4mol.L-1 with a detection limit of 2.2 × 10-7mol. L-1. Conclusion: The modified electrode has the advantages of low price, easy preparation, small amount of sample, strong anti-interference ability and can be used for determination of SN content in Zhengqing Fengtongning injection.