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目的观察神经干细胞在体外培养条件下是否有向胶质瘤细胞趋向的特性。方法 1从胎小鼠脑组织中分离出神经干细胞,并采用免疫荧光化学法检测神经干细胞标志物神经上皮干细胞蛋白(Nestin)的表达,免疫细胞化学法检测分化后细胞神经元特异性烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)的表达;2用Transwell细胞小室作神经干细胞对胶质瘤细胞趋向性实验。结果 1从胎鼠脑组织分离的细胞能形成大量悬浮的神经干细胞球,经免疫荧光化学法鉴定,巢蛋白抗体标记阳性,免疫细胞化学法鉴定分化后的神经元、胶质细胞的特异性抗原NSE及GFAP表达阳性;2神经干细胞对胶质瘤细胞趋向实验中,实验组迁移的神经干细胞数明显多于对照组及空白组,三者比较差异有统计学意义(P<0.05)。预诱导神经干细胞对胶质瘤细胞趋向实验中实验组迁移的神经干细胞数明显多于对照组及空白组,差异有统计学意义(P<0.05)。结论该研究观察到神经干细胞在体外有向胶质瘤细胞趋向的特性。预诱导状态下神经干细胞有比未诱导神经干细胞更强的向脑胶质瘤细胞趋向的趋势。
Objective To observe whether neural stem cells have the tendency to glioma cells under in vitro culture conditions. Method 1 Neural stem cells were isolated from the brain of fetal mice and the expression of neural stem cell marker neural stem cells (NSCs) was detected by immunofluorescence staining. The expressions of neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP) .2 Transwell cells were used as neural stem cells in glioma cells. Results 1 The cells isolated from fetal rat brain tissue could form a large number of suspended NSCs, which were identified by immunofluorescence staining and positive for Nestin antibody. Immunocytochemistry was used to identify differentiated neurons, glial cell specific antigen NSE and GFAP expression; 2 neural stem cells in glioma cells in the experiment, the experimental group of migrating neural stem cells were significantly more than the control group and the blank group, the difference was statistically significant (P <0.05). The number of neural stem cells migrated in pre-induced neural stem cells to glioma cells in experimental group was significantly more than that in control group and blank group, the difference was statistically significant (P <0.05). Conclusions This study observed the tendency of neural stem cells to target glioma cells in vitro. Neural stem cells tend to have a stronger tendency toward glioma cells than non-induced neural stem cells in the pre-induced state.