The receptivity of plane Poiseuille flow to local single-period micro-vibration disturbances with different phases at the top and bottom walls was investigated through direct numerical simulation of three-dimensional incompressible Navier-Stokes equations. Results show that the disturbance presents a symmetrical distribution in the spanwise direction when the micro-vibration on the wall ends, and the initial disturbance velocities and spatial distribution of the disturbance structure are different at the top and bottom walls. The disturbance’s velocity, amplitude, and high- and low-speed streaks increase with time, and the amplitude of streamwise disturbance velocity is larger than those of spanwise and vertical disturbance velocities. However, no significant Tollmien-Schlichting wave was found in the flow field. The number of disturbance vortex cores gradually increases with the disturbance area. High-speed disturbance fluid concentrates near the wall and its normal velocity largely points to the wall, while low-speed disturbance fluid largely deviates from the wall. Furthermore, the streamwise velocity profiles near the top and bottom walls both become plump because of the existence of the disturbances, and the streamwise velocity profiles show a trend of evolving into turbulent velocity profiles. The shear stress near the wall increases significantly. The local micro-vibration disturbance on the wall in plane Poiseuille flow can induce the development of a structure similar to turbulent spots. The receptivity of plane Poiseuille flow to local single-period micro-vibration disturbances with different phases at the top and bottom walls was investigated through direct numerical simulation of three-dimensional incompressible Navier-Stokes equations. Results show that the disturbance presents a symmetrical distribution in the spanwise direction when the micro-vibration on the wall ends, and the initial disturbance velocities and spatial distribution of the disturbance structures are at at the top and bottom walls. The disturbance’s velocity, amplitude, and high- and low-speed streaks increase with time, and the amplitude of streamwise disturbance velocity is larger than those of spanwise and vertical disturbance velocities. However, no significant Tollmien-Schlichting wave was found in the flow field. speed disturbance fluid concentrates near the wall and its normal velocity largely p oints to the wall, while low velocity fluid deviates from walls; while streamwise velocity profiles near the top and bottom walls both become plump because of the existence of the disturbances, and the streamwise velocity profiles show a trend of evolving The local micro-vibration disturbance on the wall in plane Poiseuille flow can induce the development of a structure similar to turbulent spots.