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干燥综合征(SS)是一种导致唾液腺及泪腺功能受损的慢性炎性疾病,本研究旨在探讨唾液蛋白电泳诊断SS的可能性。 材料和方法 43例SS患者,用39例年龄、性别、种族相匹配的健康者作对照。上午9:00~11:00收集唾液,收集前2h禁饮禁食,2%枸橼酸刺激后,持续收集10min,于-50℃下保存。采用三种电泳方法:10%十二烷基磺酸钠—聚丙烯酰胺凝胶电泳(SDS-PAGE);7.5%阴离子—聚丙烯酰胺凝胶电泳(Anionic-PAGE);
Sjogren’s syndrome (SS) is a chronic inflammatory disease that leads to impaired salivary and lacrimal gland function. This study aimed to investigate the possibility of sialoprotein electrophoresis in the diagnosis of SS. Materials and Methods Forty-three SS patients were enrolled in this study, and 39 healthy, age-matched, and sex matched subjects were used as controls. Saliva was collected at 9:00 am to 11:00 am and banned fasting for 2 hours before collection. After 2% citrate stimulation, the saliva was collected continuously for 10 minutes and stored at -50 ° C. Three electrophoresis methods were used: 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); 7.5% Anionic-PAGE;