【摘 要】
：
The Asian citrus psyllid,Diaphorina citri is the principal vector of huang-longbing,which transmits Candidatus Liberibacter asiaticus.Trehalase is a key enzyme involved in trehalose hydrolysis and plays an important role in insect growth and develop-ment.
【机 构】
：
College of Life Sciences,Gannan Normal University,Ganzhou,Jiangxi,China;National Navel Orange Engine
论文部分内容阅读
The Asian citrus psyllid,Diaphorina citri is the principal vector of huang-longbing,which transmits Candidatus Liberibacter asiaticus.Trehalase is a key enzyme involved in trehalose hydrolysis and plays an important role in insect growth and develop-ment.The specific functions of this enzyme in D.citri have not been determined.In this study,three trehalase genes(DcTre1-1,DcTre1-2,and DcTre2)were identified based on the D.citri genome database.Bioinformatic analysis showed that DcTrel-1 and DcTrel-2 are related to soluble trehalase,whereas DcTre2 is associated with membrane-bound trehalase.Spatiotemporal expression analysis indicated that DcTrel-1 and DcTrel-2 had the highest expression levels in the head and wing,respectively,and DcTre2 had high ex-pression levels in the fat body.Furthermore,DcTre1-1 and DcTre1-2 expression levels were induced by 20-hydroxyecdysone and juvenile hormone Ⅲ,but DcTre2 was unaf-fected.The expression levels of DcTre1-1,DcTre1-2,and DcTre2 were significantly up-regulated,which resulted in high mortality after treatment with validamycin.Trehalase activities and glucose contents were downregulated,but the trehalose content increased after treatment with validamycin.In addition,the expression levels of chitin metabolism-related genes significantly decreased at 24 and 48 h after treatment with validamycin.Furthermore,silencing of DcTre1-1,DcTre1-2,and DcTre2 reduced the expression levels of chitin metabolism-related genes and led to a malformed phenotype of D.citri.These re-sults indicate that D.citri trehalase plays an essential role in regulating chitin metabolism and provides a new target for control of D.citri.
其他文献
Spodoptera frugiperda(Lepidoptera:Noctuidae)is a widely distributed pest of corn.Since it invaded China in 2018,it has caused serious damage to local corn produc-tion.Chlorantraniliprole,an anthranilic diamide insecticide,has been widely used to con-trol
Wings are an important flight organ of insects and their morphogenesis de-pends on a series of cell-to-cell and cell-to-extracellular matrix interactions.Integrin as a transmembrane protein receptor mediates cell-to-cell adhesion,cell-to-extracellular ma-
Recent studies demonstrated that the Painted Lady(Vanessa cardui),a cos-mopolitan diurnal butterfly performs long-range migration between subtropical Africa and north-western Europe,covered by individuals belonging to up to six generations.Here we analyze
Lepidopteran insects produce cocoons with unique properties.The cocoons are made of silk produced in the larval tissue silk gland and our understanding of the silk genes is still very limited.Here,we investigated silk genes in the bagworm moth Eumeta vari
The evolutionary success of phytophagous insects depends on their ability to efficiently exploit plants as a source of energy for survival.Herbivorous insects largely depend on the efficiency,flexibility,and diversity of their digestive physiology and so-
Species range expansions are crucial for understanding niche formation and the interaction with the environment.Here,we studied the bumblebee Bombus haematu-rus Kriechbaumer,1870,a species historically distributed from northern Serbia through northern Ira
Diapause is an adaptation that insects have evolved to synchronize their life cycle with that of seasonal climatic changes and resources availability.However,cues for its induction are not always clear and,in some cases,a maternal effect may be involved.A
Dear Editor,rnAnopheles sinensis is the primary vector of vivax malaria in China.Given the risks posed by An.sinen-sis,many methods have been used to control this vec-tor,most commonly chemical prevention.However,the extensive use of insecticides has subj
阅读在科学学习中发挥着重要的作用,中考物理试题可以引领科学阅读.我们选取了近六年的北京市中考物理真题,对其中的科普阅读题进行分析,研究其命题特点,提出了培养学生科学阅读素养的途径.
The ladybird Harmonia axyridis is an insect that exhibits pupal attachment to plants,which facilitates development and environmental adaptation.The cremaster is highly specialized for this behavior.However,the underlying molecular regulation of the cremas