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目的探讨中介素(IMD)对大鼠体外培养心肌细胞缺氧-复氧损伤的作用。方法用大鼠H9c2心肌细胞株制作实验模型,样本分为对照组、缺氧-复氧组(缺氧1h、复氧30min)、IMD组(缺氧-复氧前30min加入10-7mol/L IMD)。采用MTT比色法检测心肌细胞活力;透射电镜观察细胞超微结构;激光共聚焦显微镜观察并测定细胞内钙离子浓度;流式细胞术测定细胞凋亡率。结果与对照组比较,缺氧-复氧组和IMD组细胞存活率显著降低,而IMD处理组明显升高细胞存活率(P<0.01);在形态学上,IMD预处理可明显减轻缺氧-复氧对大鼠心肌细胞的损伤;缺氧-复氧组细胞[Ca2+]i荧光强度和细胞凋亡率比对照组显著升高,IMD预处理可明显降低上述升高的比率(P<0.01)。结论 IMD对大鼠心肌细胞缺氧-复氧损伤有一定保护作用,提高心肌细胞存活率、减轻心肌细胞钙超载和抑制凋亡是其作用途径。
Objective To investigate the effect of interleukin-1 on hypoxia-reoxygenation injury in cultured cardiomyocytes in vitro. Methods Rat H9c2 cardiomyocytes were used to establish experimental model. The samples were divided into control group, hypoxia-reoxygenation group (hypoxia 1h, reoxygenation 30min), IMD group (10-7mol / L IMD). The viability of cardiomyocytes was detected by MTT colorimetric assay. The ultrastructure of cells was observed by transmission electron microscopy. The concentration of intracellular Ca2 + was measured by laser scanning confocal microscopy. The apoptosis rate was determined by flow cytometry. Results Compared with the control group, the cell viability was significantly decreased in hypoxia-reoxygenation group and IMD group, while IMD treatment group was significantly increased cell survival rate (P <0.01); morphologically, IMD pretreatment significantly reduced hypoxia - The effect of reoxygenation on cardiomyocyte injury in rats; The fluorescence intensity and apoptosis rate of [Ca2 +] i in hypoxia-reoxygenation group were significantly higher than those in control group, and pretreatment with IMD significantly reduced the above-mentioned rate of increase (P < 0.01). Conclusion IMD can protect rat cardiomyocytes against hypoxia-reoxygenation injury and improve cardiomyocyte viability, decrease calcium overload and inhibit apoptosis of cardiomyocytes.