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磷是能量代谢、核酸以及许多生物膜合成的重要底物。在光合作用、呼吸作用等过程中发挥了重要作用。中国大多数小麦产区的土壤存在着缺磷的问题。磷饥饿给小麦生产造成了很大损失。培育耐低磷小麦是解决这一问题的一个重要途径。在磷饥饿的过程中,哪些基因的表达发生了变化,它们是如何变化的,弄清楚这些问题对于培育转基因耐低磷小麦具有重要的意义。磷转运蛋白基因在植物吸收磷的过程中发挥着重要作用。利用RT-PCR的方法,我们从普通小麦“小偃54”中分离了两个磷转运蛋白基因TaPT8和TaPHT2;1。通过与酵母突变体互补分析表明这两个基因都能够与磷吸收功能存在缺陷的酵母突变体实现功能互补,在低磷条件下有促进酵母突变体吸收磷的作用。进一步分析表明TaPT8属于Pht1家族。TaPHT2;1属于Pht2家族。运用RQRT-PCR的方法进行分析后发现TaPT8在根中表达,受磷饥饿的诱导;TaPHT2;1主要在绿色组织中表达,受磷饥饿的抑制,受光的诱导。TaPT8可能主要参与了小麦的根从土壤中吸收磷的过程。TaPHT2;1可能在磷从细胞质向叶绿体内转运的过程中发挥了重要作用。
Phosphorus is an important substrate for energy metabolism, nucleic acids, and the biosynthesis of many membranes. In photosynthesis, respiration and other processes played an important role. The problem of phosphorus deficiency exists in the soils of most wheat-producing areas in China. Phosphorus starvation has caused a great loss of wheat production. Breeding low phosphorus-tolerant wheat is an important way to solve this problem. In the process of phosphorus starvation, the expression of some genes has changed, and how they changed. It is of great significance to clarify these problems in cultivating transgenic low-phosphorus-tolerant wheat. Phosphorus transporter genes play an important role in plant uptake of phosphorus. Using RT-PCR method, we isolated two PTX gene TaPT8 and TaPHT2 from common wheat “Xiaoyan 54” .1. Complementary analysis of yeast mutants showed that both of these two genes could function complementarily to yeast mutants with deficient P uptake, and could promote the uptake of phosphorus by yeast mutants under low P conditions. Further analysis showed that TaPT8 belongs to the Pht1 family. TaPHT2; 1 belongs to the Pht2 family. The results of RQRT-PCR showed that TaPT8 was expressed in roots and induced by phosphorus starvation. TaPHT2; 1 was mainly expressed in green tissues and was inhibited by phosphorus starvation, and was induced by light. TaPT8 may be mainly involved in the absorption of phosphorus from soil by the roots of wheat. TaPHT2; 1 may play an important role in the transport of phosphorus from the cytoplasm to the chloroplast.