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目的 探讨白细胞介素 1 β(IL 1 β)致痫或促痫的细胞内信号转导机制。 方法 动物行为学观察、免疫细胞化学方法和放射免疫分析法。 结果 行为学观察表明 :IL 1 β组大鼠有轻至中度癫痫发作 ,IL 1 β和谷氨酸(Glu)联合注射组有中至重度癫痫发作 ,IL 1受体拮抗剂 (IL 1ra)预处理可减轻或抑制癫痫发作。IL 1 β致痫或促痫时 ,大脑皮质和海马神经元Gas蛋白免疫反应明显增强 ,海马组织cAMP含量明显增高 ,IL 1ra预处理 ,则可抑制cAMP的升高。 结论 IL 1 β可能有致痫和促进Glu致痫作用 ,IL 1ra可抑制此作用。IL 1 β致痫或促痫的细胞内信号转导机制在海马区与Gas蛋白介导的AC cAMP途径可能有密切关系。
Objective To investigate the intracellular signal transduction mechanism of epilepsy or epilepsy induced by interleukin 1 β (IL 1 β). Methods Animal behavior observation, immunocytochemistry and radioimmunoassay. Results The results of behavioral observation showed that mild and moderate epileptic seizures occurred in IL 1 β group, moderate to severe epileptic seizures occurred in IL 1 β and Glu combined with IL 1 receptor antagonist (IL 1ra) Pretreatment can reduce or inhibit seizures. IL 1 β induced epilepsy or epilepsy, neurons in the cerebral cortex and hippocampus Gas protein immune response was significantly increased cAMP levels were significantly increased hippocampal IL 1ra pretreatment, can inhibit the increase of cAMP. Conclusion IL 1 β may cause epilepsy and promote Glu-induced epilepsy, IL 1ra can inhibit this effect. Intracellular signal transduction mechanism of IL-1β-induced epileptogenic or epileptogenic may be closely related to Gas protein-mediated AC cAMP pathway in hippocampus.