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近20年来,在胃粘膜中发现一种非胃蛋白酶原的蛋白酶,其命名较杂乱,如蛋白酶Ⅳ、蛋白酶2、A带、非胃蛋白酶性蛋白酶、胃组织蛋白酶(Cathepsin)、组织蛋白酶D样酸性蛋白酶等。免疫组化定位发现,人胃粘膜中有3种化学性质不同的天门冬氨酸蛋白酶,其中2种为胃蛋白酶原I(PGI)和胃蛋白酶原Ⅱ(PGⅡ),另一种即为慢泳动蛋白酶(slow moving proteinase,SMP),它是一种非胃蛋白酶原蛋白酶,因在琼脂凝胶电泳中向阳极泳动速度缓慢而命名。 SMP是从溃疡病手术时取得的胃粘膜中分离而来(其制备和纯化过程详见原文)。~(125)碘-SMP(取自抗体亲和层析法)经SDS-PAGE(Sodium dodecyl sulfate-polyacrylamide)电泳后,继以放射自显影显示2条放射活性带,其泳动介于由~(125)碘-PGⅡ和~(125)碘-PGI
In the past 20 years, a non-pepsinogenic protease was found in the gastric mucosa, and its nomenclature was disordered. For example, protease IV, protease 2, A band, non-pepsin, Cathepsin, cathepsin D Acid protease and so on. Immunohistochemical localization revealed that there are three kinds of aspartic proteases with different chemical properties in human gastric mucosa, of which two are pepsinogen I (PGI) and pepsinogen II (PG II), and the other is slow swimming The slow moving proteinase (SMP), a non-pepsinogen protease, is named for its slow anodic runout in agarose gel electrophoresis. SMP is isolated from gastric mucosa obtained during ulcer surgery (its preparation and purification are detailed in the original text). ~ (125) iodine-SMP (from antibody affinity chromatography) SDS-PAGE (Sodium dodecyl sulfate-polyacrylamide) electrophoresis, followed by autoradiography showed two radioactive bands, (125) iodine-PGII and ~ (125) iodine-PGI