【摘 要】
：
Secreted protein acidic and rich in cysteine (SPARC) is a matricellular protein highly expressed in bone tissue that acts as a chemoattractant factor promoting the arrival of prostate cancer (PCa) cells to the bone marrow.However,the contribution of SPARC
【机 构】
：
Department of Basic and Clinic Oncology, Faculty of Medicine, University of Chile, Santiago 8389100,
论文部分内容阅读
Secreted protein acidic and rich in cysteine (SPARC) is a matricellular protein highly expressed in bone tissue that acts as a chemoattractant factor promoting the arrival of prostate cancer (PCa) cells to the bone marrow.However,the contribution of SPARC during the early stages of tumor progression remains unclear.In this study,we show that SPARC is highly expressed in PCa tissues with a higher Gleason score.Through stable knockdown and overexpression of SPARC in PC3 and LNCaP cells,respectively,here we demonstrate that endogenous SPARC induces the epithelial-mesenchymal transition (EMT),decreasing E-cadherin and cytokeratin 18 and increasing N-cadherin and vimentin.Moreover,SPARC induces the expression of EMT regulatory transcription factors Snail family transcriptional repressor 1 (Snail),Snail family transcriptional repressor 2 (Slug),and zinc finger E-box binding homeobox 1 (Zeb1).In addition,SPARC knockdown in PC3 cells decreases migration and invasion in vitro,without modifying cell proliferation.Our results indicate that SPARC might facilitate tumor progression by modifying the cellular phenotype in cancer cells.
其他文献
ARAMIS is an international Phase Ⅲ trial demonstrating the beneficial role of darolutamide,a novel anti-androgen that has been found to prolong metastasisfree survival in men with nonmetastatic castration-resistant prostate cancer.Darolutamide is a novel
Structural alterations in fibroelastic components of the penile corpus cavernousum (CC) may impair its compliance,resulting in venous leakage and erectile dysfunction (ED).Our study evaluated the effectiveness of noninvasive two-dimensional shear-wave ela
For patients with moderate-to-severe erectile dysfunction,implantable penile prostheses continue to be a viable treatment.Medical device developers apply design controls during the development cycle to ensure that a product performs as intended in the fin
Up to 15％ of male infertility has an immunological origin,either due to repetitive infections or to autoimmune responses mainly affecting the epididymis,prostate,and testis.Clinical observations and epidemiological data clearly contradict the idea that th
Urologists perform the majority of vasectomies in the United States;however,family medicine physicians (FMPs) perform up to 35％.We hypothesized that differences exist in practice patterns and outcomes between urologists and FMPs.Patients who underwent a v
Pubertal development may be altered in boys with cryptorchidism and hypospadias,but existing knowledge is inconsistent.Therefore,we investigated the association between cryptorchidism and hypospadias and pubertal development in a large cohort study.Boys i
Seminal plasma is a rich source of proteins and serves as an ideal sample for proteomic analysis of male infertility.In varicoceleassociated infertility,the contributory role of seminal plasma proteins specific to unilateral and bilateral varicocele is no
The aim of this study was to validate the effectiveness of targeted microsurgical spermatic cord denervation (MSCD) of the trifecta nerve complex in comparison to traditional full MSCD with complete skeletonization of the spermatic cord in men with chroni
The sperm nucleus is prone to sustain DNA damage before and after ejaculation.Distribution of the damage is not homogeneous,and the factors determining differential sensitivity among nuclear regions have not yet been characterized.Human sperm chromatin co
Asingle armed,prospective,two-center study was conducted.with an aim of evaluating the effectiveness of the AMS 700 LGX cylinders in maintaining or increasing penile length postimplantation.The LGX has the unique ability to expand in length with inflation