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目的和方法：用Ｗｉｓｔａｒ大鼠皮下注射异丙肾上腺素（ＩＳＰ，５ｍｇ／ｋｇ）诱导心肌缺血模型。观测心肌线粒体（Ｍｉｔ）中丙二醛（ＭＤＡ）含量、Ｃａ２＋－Ｍｇ２＋－ＡＴＰ酶和Ｃａ２＋－ＡＴＰ酶活性及牛磺酸（Ｔａｕ）的影响。结果：缺血组大鼠心肌Ｍｉｔ中ＭＤＡ升高８７８３％、Ｃａ２＋－Ｍｇ２＋－ＡＴＰ酶和Ｃａ２＋－ＡＴＰ酶活性分别降低３７５６％和５０２０％（Ｐ＜０．０１）。Ｃａ２＋－Ｍｇ２＋－ＡＴＰ酶活性与ＭＤＡ含量也呈显著负相关（ｒ＝－０．８７，Ｐ＜０．０１）。Ｃａ２＋－ＡＴＰ酶活性与ＭＤＡ含量也呈显著负相关（ｒ＝－０７９，Ｐ＜０．０１）。在注射异丙肾上腺素（ＩＳＰ）前３０ｍｉｎ腹腔注射Ｔａｕ（２００ｍｇ／ｋｇ）则Ｍｉｔ中ＭＤＡ含量、Ｃａ２＋－Ｍｇ２＋－ＡＴＰ酶和Ｃａ２＋－ＡＴＰ酶活性均未见显著异常改变。结论：Ｔａｕ可能通过抑制ＭＤＡ的生成实现其保护Ｃａ２＋－ＡＴＰ酶和Ｃａ２＋－Ｍｇ２＋－ＡＴＰ酶活性的作用。
PURPOSE AND METHODS: Myocardial ischemia model was induced by subcutaneous injection of isoproterenol (ISP, 5 mg / kg) into Wistar rats. The contents of malondialdehyde (MDA), Ca2 + -Mg2 + -ATPase, Ca2 + -ATPase activity and taurine (Tau) in myocardial mitochondria (Mit) were measured. Results: The MDA in myocardium of ischemic group increased by 8783%, while the activity of Ca2 + -Mg2 + -ATPase and Ca2 + -ATP decreased 3756% and 5020% respectively (P <0.01). The Ca2 + -Mg2 + -ATPase activity was also significantly negatively correlated with MDA content (r = -0.87, P <0.01). There was also a significant negative correlation between Ca2 + -ATPase activity and MDA content (r = -0.79, P <0.01). There was no significant abnormal change of MDA content, Ca2 + -Mg2 + -ATPase and Ca2 + -ATPase activity in Mit after intraperitoneal injection of Tau (200mg / kg) 30min before injection of isoproterenol (ISP). Conclusion: Tau may protect Ca2 + -ATPase and Ca2 + -Mg2 + -ATPase activity by inhibiting the generation of MDA.