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目的：探讨红花的种内变异，为不同栽培居群红花的鉴别及优良栽培居群的选育提供依据。方法：在对不同栽培居群红花进行引种栽培的基础上，扫描电镜观察花粉粒特征，垂直平板电泳法分析细胞色素氧化酶同工酶谱带，ＵＶ、ＨＰＬＣ 测定黄色素和腺苷含量。结果：红花的孢粉特征及细胞色素氧化酶谱带特征既有种一级的共同特征，又反映了种内存在一定的变异；不同栽培居群化学成分含量之间差异的规律性与在原产地栽培时基本一致。结论：红花苞叶细胞色素氧化酶谱带可以从分子水平鉴别不同的栽培居群；红花中化学成分含量的高低，主要与遗传因素有关，同时，也受环境条件的影响。为红花的引种栽培以及优良栽培居群的选育提供依据。
Objective: To investigate the intraspecific variation of safflower, and provide basis for the identification of safflower in different cultivated populations and the breeding of excellent cultivated populations. METHODS: Based on the introduction and cultivation of cultivars of cultivar Carthamus tinctorius, scanning electron microscopy (SEM) was used to observe the characteristics of pollen grains. The vertical band plate electrophoresis was used to analyze the cytochrome oxidase isoenzyme bands. UV and HPLC were used to determine the contents of yellow pigment and adenosine. RESULTS: The characteristics of sporo-pollen and cytochrome oxidase bands of safflower were common to both species and reflected a certain variation within the species; the regularity of the differences between chemical components in different cultivation populations was It is basically the same when cultivated in the country of origin. Conclusion: The cytochrome oxidases of red oak leaves can be used to identify different cultivated populations from the molecular level. The content of chemical constituents in safflower is mainly related to genetic factors. At the same time, it is also affected by environmental conditions. It provides the basis for the introduction and cultivation of safflower and the breeding of excellent cultivation populations.