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以受体杨树‘107号’和转入NTHK1(Nicotiana tabacum histidine kinase-1)基因的‘18-1’及‘18-4’的水培苗为材料,不同浓度的NaCl胁迫12d后,发现‘18-1’及‘18-4’的根长和根重显著大于‘107号’。以离体根段为材料,在400mmol·L-1NaCl溶液中胁迫60min后,‘107号’的K+外渗量比‘18-1’和‘18-4’分别高49.34%和19.68%;在400mmol·L-1KCl或NaCl胁迫30min,‘107号’的电解质外渗率(REL)显著大于‘18-1’和‘18-4’;等渗的30%PEG对离体根段的REL影响很小。在400mmol·L-1NaCl溶液中添加200～400mmol·L-1的KNO3或KCl能显著增加离体根段的REL,并使不同基因型的REL差异更大。这表明,测定离体根段的K+外渗量和REL可以快速鉴定不同基因型杨树的耐盐潜力。
Using hydroponic culture of ’107’ and ’18-1’ and ’18 -4 ’of NTHK1 (Nicotiana tabacum histidine kinase-1) as materials, we found that under NaCl stress of different concentrations for 12 days, The root length and root weight of ’18-1 ’and ’18-4’ were significantly larger than that of ’107’. The exudation rate of K + of ’107’ was 49.34% and 19.68% higher than that of ’18-1 ’and ’18 -4’, respectively, after the roots were treated with 400mmol·L-1 NaCl for 60min. Electrolyte extravasation rate (REL) of ’107’ was significantly greater than that of ’18-1 ’and ’18-4’ under 400 mmol·L-1 KCl or NaCl stress for 30 min. The effect of isotonic 30% PEG on REL of isolated root segments Very small. The addition of 200 ~ 400mmol·L-1 KNO3 or KCl in 400mmol·L-1NaCl solution can significantly increase the REL of ex vivo roots and make the difference of REL of different genotypes greater. This indicates that the determination of exudative K + extravasation volume and REL can quickly identify the salt tolerance potential of different genotypes of poplar.