先天性感染鼠弓形体病能使新生儿或婴儿产生严重后果。然而,对妊娠期妇女筛选弓形体病尚存争议,且既往弓形体病的产前诊断方法复杂、费时。为此,作者以聚合酶链反应(PCR)对羊水进行了弓形体病的诊断,并用传统的方法(将羊水和胎血接种于鼠、羊水组织培养及胎血特异性IgM鉴定)相比较。 Congenital infection with toxoplasmosis can have serious consequences for newborns or infants. However, the screening of toxoplasmosis in pregnant women is controversial and the prenatal diagnosis of previous toxoplasmosis is complex and time-consuming. To do this, the authors used polymerase chain reaction (PCR) to diagnose the toxoplasmosis of amniotic fluid and compared it with the traditional method (inoculation of amniotic fluid and fetal blood in mice, amniotic fluid cultures and fetal blood-specific IgM identification).