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Aim:To investigate whether the increasing CD40-CD40 ligand system is relatedto restenosis in patients after percutaneous coronary intervention(PCI).Methods:Twenty normal controls and 120 patients with PCI were investigated.The expres-sion of CD40 and the CD40 ligand(CD40L)on platelets was analyzed by indirect-immunofluorescence flow cytometry.The serum level of soluble CD40L(sCD40L)and C-reactive protein(CRP)was determined by commercially available enzymelinked immunosorbent assay.Restenosis was observed in 120 patients within a 6month follow-up period after PCI surgery.Results:Restenosis occurred in 29patients(24.2%).Patients who developed restenosis showed higher levels ofCD40-CD40L system compared with non-restenotic patients before PCI.Allrestenotic patients showed a significant increase in CD40[66.9±4.8,mean fluores-cence intensity(MFI)]and CD40L(14.9±1.8,MFI)co-expression on platelets aswell as sCD40L(13.7±1.7 ng/mL)compared with non-restenotic patients and con-trols during the 6 month follow-up period(P<0.01).Elevated sCD40L and CD40Lwere significantly correlated with the serum level of CRP after percutaneoustransluminal coronary angioplasty and with lumen loss during the 6 month follow-up period.Conclusion:The level of CD40L was associated with late restenosisafter PCI indicating that restenosis is an inflammatory disorder.
Aim: To investigate whether the CD40-CD40 ligand system is related to restenosis in patients after percutaneous coronary intervention (PCI). Methods: Twenty normal controls and 120 patients with PCI were investigated. The expres-sion of CD40 and the CD40 ligand (CD40L ) on platelets was analyzed by indirect-immunofluorescence flow cytometry. The serum level of soluble CD40L (sCD40L) and C-reactive protein (CRP) was determined by commercially available enzyme linked immunosorbent assay. Restenosis was observed in 120 patients within a 6month follow-up Patients who developed restenosis showed higher levels of CD40-CD40L system compared with non-restenotic patients before PCI. All of the patients showed a significant increase in CD40 [66.9 ± 4.8, mean fluores-cence intensity (MFI)] and CD40L (14.9 ± 1.8, MFI) co-expression on platelets aswell as sCD40L (13.7 ± 1.7 ng / mL) compared with non-restenotic patients and con-trols during th E 6 months follow-up period (P <0.01). Elevated sCD40L and CD40Lwere significantly correlated with the serum level of CRP after percutaneoustransluminal coronary angioplasty and with lumen loss during the 6 month follow-up period. Conlusion: The level of CD40L was associated with late restenosis after PCI indicates that restenosis is an inflammatory disorder.