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目的 研究原发性肝细胞癌中p16蛋白的表达及其基因的缺失情况 ,探讨p16基因与肝癌的关系。方法 采用回顾性调查研究方法 ,收集我院原发性肝癌 2 5例 ,用免疫组织化学SP法、聚合酶链反应 (PCR)方法检测肝癌及癌旁组织中p16蛋白的表达及其基因的扩增情况。结果2 5例肝癌中 ,p16蛋白阴性表达率为 6 8% (17/ 2 5 ) ,癌旁组织中p16蛋白阴性表达率为 4% (1/ 2 5 )。两者差异有显著意义 (P <0 0 1)。p16蛋白的表达与肝癌分化程度关系密切 (P <0 0 5 ) ,而与癌灶体积、甲胎球蛋白 (AFP)值的差异无显著意义 (P >0 0 5 )。p16基因在癌组织中的缺失率 (2 8% ,7/ 2 5 )显著高于癌旁组织 (4 % ,1/ 2 5 ) (P <0 0 5 )。p16基因缺失与肝癌分化程度、癌灶体积、AFP值无关 (P >0 0 5 )。结论 p16基因失活与肝癌发生关系密切。除了基因缺失之外 ,还有其他引起p16基因失活的因素存在。p16蛋白的检测可能是预测病人预后的一个指标 ,但尚需进一步证实。
Objective To study the expression of p16 protein and the deletion of gene in primary hepatocellular carcinoma, and to explore the relationship between p16 gene and hepatocellular carcinoma. Methods A retrospective study was conducted to collect 25 cases of primary hepatocellular carcinoma in our hospital. The expression of p16 protein and its gene amplification were detected by SP immunohistochemical method and polymerase chain reaction (PCR) method. Increased situation. Results The negative expression rate of p16 protein was 6 8% (17/25) in 25 cases of hepatocellular carcinoma. The negative expression rate of p16 protein was 4% (1/25) in adjacent tissues. There was a significant difference between the two (P < 0 01). The expression of p16 protein was closely related to the degree of differentiation of hepatocellular carcinoma (P <0 05), but it was not significantly different from the volume of tumor foci and alpha-fetoprotein (AFP) values (P > 0.05). The deletion rate of p16 gene in cancer tissues (28%, 7/25) was significantly higher than that in adjacent tissues (4%, 1/25%) (P < 0.05). The deletion of p16 gene was not associated with the degree of differentiation, tumor volume, and AFP value (P > 0.05). Conclusion The inactivation of p16 gene is closely related to the occurrence of liver cancer. In addition to gene deletion, there are other factors that cause the inactivation of the p16 gene. The detection of p16 protein may be an indicator of the prognosis of the patient, but further confirmation is needed.