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将离体细胞在实验室中进行长期培养的组织细胞培养技术,已广泛应用于医学科学的研究。人羊膜细胞WISH株(以下简称Wish细胞)是目前用于测定人干扰素抗病毒活性的常用检测细胞株,其培养的好坏直接关系干扰素的研究、生产和测定,为此,笔者将Wish细胞在液氮(-196℃)中冻存及复苏培养的一些经验简介如下。
Tissue cell culture technology that long-term cultured ex vivo cells in the laboratory has been widely used in medical science. Human amniotic WISH strain (hereinafter referred to as Wish cells) is currently used to determine the human interferon antiviral activity commonly used detection of cell lines, the culture is directly related to the quality of interferon research, production and determination, for which I author Wish Some of the experiences of cryopreservation and resuscitation of cells in liquid nitrogen (-196 ° C) are summarized below.