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目的 :观察前列地尔与川芎嗪 (LT )、黄芪(AM ) 3药合用对大鼠心肌缺血再灌注损伤的保护作用。方法 :采用在体大鼠开胸结扎冠状动脉左室支 30min后 ,松扎再灌注 60min造成心肌缺血再灌注模型并以 0 .9%氯化钠注射液为模型对照 ,观察 3药 :前列地尔 31.2 5μg·kg- 1,川芎嗪 2 5mg·kg- 1,黄芪 4 15mg·kg- 1合用对再灌注心肌组织中超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶(GSH PX)活力 ,丙二醛 (MAD)、Ca2 +含量及血清肌酸磷酸激酶同功酶 (CK MB)含量的变化 ,并同时心电图监测心律失常情况。结果 :与模型对照组相比3药合用可提高再灌注心肌组织中SOD ,GSH PX活力 ,差异有非常显著意义 (P <0 .0 1) ;并能降低再灌注心肌组织中MDA ,Ca2 +含量及血清CK MB含量 ,差异有非常显著意义 (P <0 .0 1) ;防止再灌注室性心律失常的发生 ,缩短心律失常的维持时间 ,降低ST段抬高的程度 ,差异有非常显著意义 (P <0 .0 1)。结论 :前列地尔与LT ,AM合用在保护心肌缺血再灌注损伤中有显著的作用 ,其保护作用主要与清除自由基有关
Objective: To observe the protective effects of alprostadil and ligustrazine (LT) combined with astragalus (AM) 3 on myocardial ischemia-reperfusion injury in rats. Methods: The left ventricular branch of coronary artery was ligated for 30min in the thoracotomy of rats, then the myocardial ischemia-reperfusion model was induced by reperfusion of mandibular rectum for 60min and the 0.9% sodium chloride injection was taken as the model control. 31.6 μg · kg-1, ligustrazine 25 mg · kg-1 and Astragalus membranaceus 4 15 mg · kg-1, respectively. The effects of combined treatment on the activities of superoxide dismutase (SOD), glutathione peroxidase PX) activity, malondialdehyde (MAD), Ca2 + content and serum creatine phosphokinase isoenzyme (CK MB) levels were measured. Cardiac arrhythmia was monitored by electrocardiogram at the same time. Results: Compared with the model control group, the combination of three drugs could increase the activities of SOD and GSH PX in myocardial tissue after reperfusion, the difference was significant (P <0.01); and the content of MDA and Ca2 + Content and serum CK MB content, the difference was significant (P <0. 01); to prevent reperfusion ventricular arrhythmias, shorten the maintenance of arrhythmia and reduce the degree of ST elevation, the difference was significant Significance (P <0 .01). Conclusion: Combination of alprostadil with LT and AM has a significant effect on the protection of myocardial ischemia-reperfusion injury, and its protective effect is mainly related to the scavenging of free radicals