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目的:从分子生物学水平探究微卫星的不稳定性(MSI)与杂合性缺失(LOH)在喉鳞状细胞癌发病机制中的意义。方法:选择3号,5号及11号染色体的3个微卫星位点采用PCR和聚丙烯酰胺凝胶电泳-硝酸银染色方法对40例喉鳞状细胞癌患者手术切除的癌组织及癌旁正常组织进行微卫星分析。结果:40例喉鳞状细胞癌中,35例(87.5%)分别有1～3个微卫星位点发生MSI或LOH。微卫星异常改变发生率最高的位点为D5s592,占70%(28/40);其次是D3s1228位点,占52.5%(21/40)。结论:在3p14区域及5q23区域附近的抑癌基因参与致癌机制,D3s1228和D5s592的微卫星改变与喉鳞状细胞癌的临床分期相关。
Objective: To explore the significance of microsatellite instability (MSI) and loss of heterozygosity (LOH) in the pathogenesis of laryngeal squamous cell carcinoma at molecular biology level. Methods: Three microsatellite loci on chromosomes 3, 5 and 11 were selected for PCR. Polyacrylamide gel electrophoresis-silver nitrate staining was used to evaluate the clinical data of 40 cases of laryngeal squamous cell carcinoma Normal tissue microsatellite analysis. Results: In 40 cases of laryngeal squamous cell carcinoma, MSI or LOH occurred in 1 ~ 3 microsatellite loci in 35 cases (87.5%). The site with the highest incidence of microsatellite anomalies was D5s592, accounting for 70% (28/40), followed by D3s1228 (52.5%, 21/40). CONCLUSION: The tumor suppressor genes in the 3p14 and 5q23 regions are involved in carcinogenesis. The microsatellite alterations of D3s1228 and D5s592 are correlated with the clinical stage of laryngeal squamous cell carcinoma.