“五四○六”菌种的生产过程中,一般连续繁殖3—4代以后,即迅速退化,用已退化的菌种制造母剂或堆肥,不但生产慢,而且质量差。最近我们从现有的实际条件出发,为便于在各公社和生产队推广,经过多次研究实验,成功地寻求到一种简易复壮的方法(即更换培养基法),具体做法: 将繁殖了2—3代的(以琼脂、土豆做培养基的)菌种接种到“饼土培养基”或 In the production process of “5460” bacteria, generally 3-4 generations of continuous breeding, that is, the rapid degeneration, with degenerated strains of mother or composting, not only slow production, but also poor quality. In light of the current actual conditions, we have recently succeeded in finding a simple and rejuvenating method (ie, the medium-changing method) for the convenience of promotion in various communes and production teams by conducting numerous research experiments. The specific approach is to popularize 2-3 generations of (in agar, potato culture medium) inoculation to “cinnamon medium” or