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采用ＫｙｔｅＤｏｏｌｉｔｔｌｅ氨基酸亲水性标准及Ｂａｉｒｏｃｈ的ＧＧＢＳＭ方案对人Ｃ５ａＲ分子的亲水性基序、Ｂ细胞表位及二级结构进行了预测，并用吴氏建立的Ｂ细胞表位预测方法进行评述。结果显示，不同的预测方案所得结果一致，人Ｃ５ａＲ分子中，构成螺旋结构，伸展构象及无规卷曲的氨基酸残基数比例分别为３３．７％，３４．６％，３１．７％。三个高亲水性区域为第１５～１８位的ＤＤＫＤ，第１９５～２００位的ＤＫＲＲＥＲ和第３３０～３３３位的ＲＥＳＫ，其Ａｈ值分别为３．０、３．０、２．３２。其平均抗原性指数ＡＩ值分别为０．０６４、０．０９１、０．０７０。Ｎ－端第９～３０氨基酸序列的ＡＩ＝０．０４２，是Ｂ细胞优势表位区，以此制作短肽单克隆抗体是可行的。
The hydrophilic motifs, B cell epitopes and secondary structures of human C5aR molecules were predicted using KyteDoolittle Amino Acid Hydrophilicity Standard and Bairoch’s GGBSM protocol. The B cell epitope predicting method established by Wu was reviewed. The results showed that the results obtained by different prediction schemes were consistent. The proportion of amino acid residues in human C5aR molecules that constituted helical structure, stretch conformation and random coil were 33.7%, 34.6% and 31.7% respectively. The three highly hydrophilic regions are DDKD at positions 15-18, DKRRER at positions 195-200 and RESK at positions 330-333, with Ah values of 3.0, 3.0 and 2.32, respectively. The average antigenicity index AI values were 0.064,0.091,0.070. N-terminal 9 to 30 amino acid sequence of AI = 0.042, B cell is the dominant epitope region, in order to produce short peptide monoclonal antibody is feasible.