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The hydrolysis technology and reaction kinetics for amino acids production from fish proteins in sub-critical water reactor without catalysts were investigated in a reactor with volume of 400 ml under the conditions of reaction temperature from 180-320oC, pressure from 5-26 MPa, and time from 5-60 min. The quality and quantity of amino acids in hydrolysate were determined by bioLiquid chromatography, and 17 kinds of amino acids were obtained. For the important 8 amino acids, the experiments were conducted to examine the effects of reaction tem-perature, pressure and time on amino acids yield. The optimum conditions for high yield are obtained from the ex-perimental results. It is found that the nitrogen and carbon dioxide atmosphere should be used for leucine, isoleu-cine and histidine production while the air atmosphere might be used for other amino acids. The reaction time of 30 min and the experimental temperature of 220oC, 240oC and 260oC were adopted for reaction kinetic research. The total yield of amino acids versus reaction time have been examined experimentally. According to these experimental data and under the condition of water excess, the macroscopic reaction kinetic equation of fish proteins hydrolysis was obtained with the hydrolysis reaction order of 1.615 and the rate constants being 0.0017,0.0045 and 0.0097 at 220oC, 240oC and 260oC respectively. The activation energy is 145.1 kJ?mol-1.
The hydrolysis technology and reaction kinetics for amino acids production from fish proteins in sub-critical water reactor without catalysts were investigated in a reactor with volume of 400 ml under the conditions of reaction temperature from 180-320oC, pressure from 5-26 MPa, and The quality and quantity of amino acids in hydrolysate were determined by bio-Liquid chromatography, and 17 kinds of amino acids were obtained. For the important 8 amino acids, the experiments were conducted to examine the effects of reaction tem- The optimum conditions for high yield are obtained from the ex-perimental results. It is found that the nitrogen and carbon dioxide atmosphere should be used for leucine, isoleu-cine and histidine production while the air The reaction time of 30 min and the experimental temperature of 220oC, 240oC and 260oC were adopted for reaction kinetic research. The total yield of amino acids versus reaction time have been examined experimentally. According to these experimental data and under the condition of water excess, the macroscopic reaction kinetic equation of fish protein hydrolysis was obtained with the hydrolysis reaction order of 1.615 and the rate constants being 0.0017, 0.0045 and 0.0097 at 220oC, 240oC and 260oC respectively. The activation energy is 145.1 kJ? Mol-1.