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目的:观察链脲佐菌素(streptozotocin,STZ)诱导的糖尿病大鼠早期边缘系统的扣带回、背侧丘脑、下丘脑和杏仁体中生长相关蛋白(growth-associated protein,GAP-43)的变化,探讨GAP-43在糖尿病脑病发病中的重要作用。方法:SD雄性大鼠20只随机分为正常组及糖尿病组,用STZ复制糖尿病动物模型,饲养7 d后测血糖,4周后应用免疫组织化学方法观察糖尿病组和正常组大鼠的扣带回、背侧丘脑、下丘脑和杏仁体GAP-43的表达情况。结果:糖尿病组大鼠边缘系统的扣带回、背侧丘脑、下丘脑和杏仁体的GAP-43表达增高。结论:糖尿病大鼠早期边缘系统GAP-43表达增加,反映了神经组织对机体糖代谢紊乱的适应性改变过程,提示在糖尿病的早期已经发生了神经组织的改变。
OBJECTIVE: To observe the expression of growth-associated protein (GAP-43) in the cingulate gyrus, dorsal thalamus, hypothalamus and amygdala in diabetic rat induced by streptozotocin (STZ) Change, to explore the important role of GAP-43 in the pathogenesis of diabetic encephalopathy. Methods: Twenty male Sprague-Dawley rats were randomly divided into normal group and diabetic group. STZ-induced diabetic animal models were used to measure blood glucose after 7 days of rearing. After 4 weeks, immunohistochemistry was used to observe the cingulate lesions in diabetic rats and normal rats Back, dorsal thalamus, hypothalamus and amygdala GAP-43 expression. RESULTS: GAP-43 expression was increased in the cingulate gyrus, dorsal thalamus, hypothalamus and amygdala of diabetic rats. CONCLUSION: The increased expression of GAP-43 in the early marginal system of diabetic rats reflects the adaptive changes of nerve tissue to the disorder of glucose metabolism in the diabetic rats, suggesting that nerve tissue changes have occurred in the early stage of diabetes.