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本文采用体外混合淋巴细胞反应(Mixed Lymphocyte Reaction,MLR)与体内细胞过继转移试验进一步对巨噬细胞(Mφ)在新生小鼠移植耐受维持和崩溃中的作用进行了研究。体外MLR中,耐受小鼠的脾脏单层粘附细胞不能重建去粘附细胞的同系正常鼠的MLR,相反呈现出抑制作用。另一方面,正常小鼠的脾脏与腹腔单层粘附细胞却能恢复同系耐受鼠脾细胞的MLR至正常水平。体内实验中,过继转移正常小鼠腹腔Mφ 能恢复同系耐受鼠(切除或未切除脾脏、淋巴结)对同种心移植物的排斥反应。这种作用明显地见于新移植心,而对已耐受的移植心则作用不明显。本文结果提示,耐受鼠Mφ 递呈耐受原功能的缺陷以及Mφ 抑制作用可能是免疫耐受得以维持的重要原因,一旦功能恢复(体内外重建)则耐受崩溃。
In this study, the role of macrophages (Mφ) in maintaining tolerance and collapse of neonatal mice was studied by mixed lymphocyte reaction (MLR) in vitro and adoptive transfer assay in vivo. In in vitro MLR, the monolayer adherent cells in the spleen of the tolerant mice failed to reconstruct the MLR of normal mice that adhered to the cells, but showed an inhibitory effect on the contrary. On the other hand, the normal mouse spleen and abdominal monolayer adherent cells can restore the MLR of normal mice tolerant spleen cells to normal levels. In vivo, adoptive transfer of normal mice to Mφ in the peritoneal cavity can restore the rejection of homograft tolerant rats (resected or not removed spleen, lymph nodes) on allograft rejection. This effect is clearly seen in the new transplant heart, and has no effect on the transplant heart has been tolerated. Our results suggest that the tolerance of murine Mφ tolerance to protential function defects and Mφ inhibition may be an important reason for the maintenance of immune tolerance, once the functional recovery (in vitro reconstruction) are resistant to collapse.